كتابة النص: الأستاذ الدكتور يوسف أبو العدوس - جامعة جرش قراءة النص: الدكتور أحمد أبو دلو - جامعة اليرموك مونتاج وإخراج : الدكتور محمد أبوشقير، حمزة الناطور، علي ميّاس تصوير : الأستاذ أحمد الصمادي الإشراف العام: الأستاذ الدكتور يوسف أبو العدوس
فيديو بمناسبة الإسراء والمعراج - إحتفال كلية الشريعة بجامعة جرش 2019 - 1440
فيديو بمناسبة ذكرى المولد النبوي الشريف- مونتاج وإخراج الدكتور محمد أبوشقير- كلية تكنولوجيا المعلومات
التميز في مجالات التعليم والبحث العلمي، وخدمة المجتمع، والارتقاء لمصاف الجامعات المرموقة محليا واقليميا وعالميا.
المساهمة في بناء مجتمع المعرفة وتطوره من خلال إيجاد بيئة جامعية، وشراكة مجتمعية محفزة للابداع، وحرية الفكر والتعبير، ومواكبة التطورات التقنية في مجال التعليم، ومن ثم رفد المجتمع بما يحتاجه من موارد بشرية مؤهلة وملائمة لاحتياجات سوق العمل.
تلتزم الجامعة بترسيخ القيم الجوهرية التالية: الإلتزام الإجتماعي والأخلاقي، الإنتماء،العدالة والمساواة، الإبداع، الجودة والتميّز، الشفافية والمحاسبة، الحرية المنظبطة والمستقبلية.
2017 PhD in analytical chemistry (University of Duisburg-Essen, Essen, Germany), Very good rank (with great honor).
2011 MSc in chemistry (University of Jordan, Jordan), with rank of 3.06.
2004 BSc in chemistry (University of Petra, Jordan), with 3.25rank.
09/2019 present: Assistant professor in facility of pharmacy, Jerash university.
09/2018 to 09/2019: Researcher in monitoring and assessing directorate, Ministry of Environment, Jordan. Including management of landfills and air pollutants monitoring.
05/2017 to 07/2018: (part time) Consulting in Elriah research center, liver research institute, Egypt. Including new bioanalytical lab establishment, bioequivalence studies direction, analytical instruments demonstration.
01/2015 to 03/2017: PhD research attendance, following a multi objective project, titled thesis with ‘Determination of Nicotine, Cotinine and Nicotine N-Oxide in Human Blood, Plasma, Urine, Semen and Sperm by LC-Orbitrap MS: Application to Clinical Study’.
08/2013 to 10/2014: Product and application specialist for Waters Inc.
in Dafco, Saudi Arabia.
07/2004 to 07/2013: Lab management in Jordan center for pharmaceutical research, Amman, Jordan. Including bio-analytical methods development and validation, bioequivalence studies direction.
Nicotine (Nic) distribution in human fluids and tissues has a deleterious effect on human health. In addition to its poisoning profile, Nic may contribute to the particular impact of smoking on human reproduction. Although present in seminal fluid, still nobody knows whether nicotine is available in sperm or not. Herein, we developed and validated a new bioanalytical method, for simultaneous determination of Nic, cotinine (Cot), and nicotine N'-oxide (Nox) in human plasma, semen, and sperm by LC-ESI-orbitrap-MS. Blood and semen samples were collected from 12 healthy smoking volunteers in this study. Sperm bodies were then separated quantitatively from 1 mL of semen samples by centrifugation. The developed method was fully validated for plasma following European and American guidelines for bioanalytical method validation, and partial validation was applied to semen analysis. Plasma, semen, and sperm samples were treated by trichloroacetic acid solution for protein direct precipitation in single extraction step. The established calibration range for Nic and Nox in plasma and semen was linear between 5 and 250 ng/mL, and for Cot between 10 and 500 ng/mL. Nic and Cot were detected in human sperm at concentrations as high as in plasma. In addition, Nox was present in semen and sperm but not in plasma. Graphical abstract Nicotine correlation between plasma and semen a; Nicotine correlation between semen and sperm c; Cotinine correlation between plasma and semen b; Cotinine correlation between semen and sperm d.
Nicotine-diet interactions have a particular importance on human health. Some food substances are subject to change hepatic CYP2A6 metabolism rate for nicotine and its levels in smokers consequently. This study investigates the effect of pomegranate and licorice drinks on nicotine metabolism, by a new developed and validated method for simultaneous determination of nicotine with its major metabolites (cotinine and nicotine N-oxide) in human urine, utilizing LC ESI-orbitrap-MS. Twenty-four Jordanian healthy and smoker volunteers were participated in two equal groups, crossover design for each of pomegranate and licorice test drink. In the study periods each group assigned either to drink test juice three times a day or to be avoided from test drink for 7 successive days, and then both groups switched their drink treatment in subsequent period. Early morning urine samples were collected from all volunteers after each period. Nicotine metabolism rate was evaluated from nicotine/cotinine and nicotine/nicotine N-oxide ratios in urine. A consistent trend of increase in metabolism rate for nicotine was observed from urine analysis under pomegranate or licorice drink conditions compared to control conditions. Pomegranate and licorice drinks are increasing the metabolism rate for nicotine in terms of induction effect for hepatic cytochrome p450 enzymes.
Background: Dried blood spot (DBS) have been applied in many application as alternate analytical solution for limitations and challenges in conventional methods, and validated methods demand by DBS technique are still required in nicotine (Nic) research area.Aim: The development and validation of a new bioanalytical method for simultaneous determination of Nic and cotinine (Cot) in human blood using DBS and LC-Orbitrap MS technique.Methods: The DBS was punched at 6.35 mm diameter and extracted by 10% w/v of trichloroacetic acid solution, containing Nic-d3 as an internal standard (IS). The extracted samples were then injected into a Kinetex-C18 column and eluted by mobile phase of methanol:water:formic acid (10:90:0.001, v/v/v). Chromatographic effect for DBS was investigated by decentralized disk punching (peripheral area). The developed method was validated according to European and American guidelines for bioanalytical method validation.Results: Nic, Cot and IS were detected accurately by Orbitrap-MS using heated-ESI source at positive ions m/z of 163.1235, 177.1028 and 166.1423, respectively. The established calibration ranges for Nic and Cot were linear between 5-250 ng/mL and 10-500 ng/mL, respectively. Within- and between-run measurements accuracy for Nic and Cot were all higher than 80 % for LLOQ and 85 % for QC levels, and the measurements precision for Nic and Cot were all within 15 %.Conclusion: The developed method for Nic and Cot determination in human blood was successfully validated using DBS LC-Orbitrap MS technique. Nic and Cot investigation was not affected by DBS’s chromatographic influence.
Enalapril is an angiotensin-converting enzyme inhibitor used for treatment of hypertension and chronic heart disease. Enalaprilat is its active metabolite responsible for the activity. This study aimed to develop and validate a method for enalapril and enalaprilat analysis and to determine the bioequivalence of two tablet formulae of enalapril. LC-MS/MS bioanalytical method was developed and validated and then applied to evaluate the bioavailability of two enalapril formulae. Antihyperglycemic sitagliptin was used as internal standard (IS). The method was accurate for the within- and between-days analysis, and precise CV% was <5%, being linear over the calibration range 1.0-200.0 ng/ml. Stability was >85% and the LOD was 0.907 and 0.910 ng/ml for enalapril and enalaprilat, respectively, and LLOQ was 1 ng/ml. The pharmacokinetic parameters Cmax, tmax, AUC0-72, and AUC0-∞ values of enalapril and enalaprilat of the two formulae were calculated and nonsignificant differences were found. A linearity, specific, accurate, and precise method was developed and applied for the analysis of enalapril and enalaprilat in human plasma after oral administration of two formulae of enalapril 20 mg tablets in healthy volunteers. Depending on the statistical analysis it was concluded that the two enalapril formulae were bioequivalent.
A new multi-residue analytical method for simultaneous determination of four antibiotic resi-dues (doxycycline (DOX), enrofloxacin (EN), ciprofloxacin (CP) chloramphenicol (CAM) in cow’s milk has developed and validated by liquid chromatography-tandem mass spectrometry, was successfully ap-plied to investigate commercially available cow’s milk in Jordan. The analytes including etoricoxib as IS were extracted using liquid-liquid extraction and separated from their matrix chromatographically by using Fortis UniverSil Cyano column (50 × 2.1 mm, 5 μm), eluted by a mobile phase of 0.5 mM ammo-nium chloride /methanol /formic acid (35 : 65 : 0.08%, v/v) and delivered isocratically at a constant flow rate of 0.4 mL/min for total LC run time of 1 min. Twenty-six cow’s milk samples from different brands of dry powder milk, long shelf-life milk, and raw untreated milk were collected randomly from the Jorda-nian market and analyzed in the triplicate analysis. The calibration curve was linear within the dynamic range of 10-1000 ng/mL in spiked milk for each analyte and the correlation coefficients were greater than 0.9973 for all calibration curves during validation. The internal standard-normalized matrix effects extend from 0.901 to 1.11. The intra-assay and inter-assay precision normalized matrix effects extend from 0.901 to 1.11. The within-day and between-day precision ranged from 2.60-12.71% and 2.68-12.66%, respectively, and the relative error of accuracy does not exceed 15%. The results obtained are less than the approved stated regulatory guidelines and all samples screened were found to be free of any of the antibiotics tested.
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